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Somatostatin is a neuropeptide with modulatory effects on the immune system and the function of synovial cells; it has antiangiogenic and antiproliferative properties. This study aimed to evaluate the clinical, histological, and a...
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Somatostatin is a neuropeptide with modulatory effects on the immune system and the function of synovial cells; it has antiangiogenic and antiproliferative properties. This study aimed to evaluate the clinical, histological, and articular tissue cytokine mRNA response to somostatin treatment in rat adjuvant-induced arthritis (AIA).
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Rheumatoid arthritis (RA) is a chronic inflammatory disease that mainly targets the synovial membrane, cartilage and bone. It affects 1% of the population and is associated with significant morbidity and increased mortality. Se is...
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Rheumatoid arthritis (RA) is a chronic inflammatory disease that mainly targets the synovial membrane, cartilage and bone. It affects 1% of the population and is associated with significant morbidity and increased mortality. Se is an essential trace element with antioxidant properties and the ability to modulate the immune responses. SelemaxReg. is an inactive yeast (Saccharomyces cerevisiae) enriched with organic Se. The aim of the present study was to investigate the effects of SelemaxReg. administration in models of an antigen-induced arthritis (AIA) in C57BL/6 mice, and of an adjuvant-induced arthritis (AdIA) in Holtzman rats. As control, the animals were treated with the same inactivated yeast species that was not enriched for Se. In the AIA model, treatment with different doses of SelemaxReg. (0.01, 0.1, 1 and 10% added to food) significantly decreased the number of inflammatory cells recruited to the knee cavity, essentially by reducing the number of neutrophils. Levels of proinflammatory cytokines, including TNF- alpha, IL-1 beta and chemokine (C-X-C motif) ligand 1/keratinocyte chemoattractant (CXCL1/KC), were also reduced in the peri-articular tissue of mice treated with SelemaxReg. at the tested dose (1%). In the AdIA model in rats, SelemaxReg. treatment decreased paw oedema and hypernociception. This reduction was associated with inhibition of the influx of proinflammatory cells. Therefore, treatment with SelemaxReg. is associated with amelioration of several inflammatory and functional parameters in models of arthritis, suggesting that this Se-enriched yeast should be evaluated further in patients with RA. Copyright copyright The Authors 2012.
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Aim: Rheumatoid arthritis is a chronic inflammatory autoimmune disorder with multi-factorial factors influencing disease alleviation in synovial joints. The aim of this study was to investigate the anti-arthritic efficacy of trika...
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Aim: Rheumatoid arthritis is a chronic inflammatory autoimmune disorder with multi-factorial factors influencing disease alleviation in synovial joints. The aim of this study was to investigate the anti-arthritic efficacy of trikatu, a herbal compound, on biochemical and immunological complications in adjuvant-induced arthritic rats.
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There is increasing body of evidence documenting the involvement of angiotensin II in inflammatory diseases. Moreover the up-regulation of angiotensin II AT1 receptors in the synovium of rheumatoid arthritis patients has been prev...
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There is increasing body of evidence documenting the involvement of angiotensin II in inflammatory diseases. Moreover the up-regulation of angiotensin II AT1 receptors in the synovium of rheumatoid arthritis patients has been previously described. This study aimed at investigating the anti-inflammatory effect of losartan, the selective angiotensin II AT 1 receptor blocker, and comparing the efficacy of methotrexate alone and in combination with losartan in adjuvant arthritis in rats. Twelve days post adjuvant injection, Sprague-Dawley rats were treated with methotrexate (1 mg/kg/week), losartan (20 mg/kg/day) and their combination for 15 days. Severity of arthritis was assessed by hind paw swelling, arthrogram scores. Serum was analyzed for measurement of albumin, C-reactive protein (CRP), nitrite/nitrate concentrations, interleukin 1β (IL-1β), tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF), aspartate transaminase (AST) and alanine transaminase (ALT). Histopathological examination was done for hind paws and livers. Methotrexate and losartan monotherapies significantly reduced all parameters of inflammation and arthritis with better results in the methotrexate group except for the transaminases where losartan caused more significant reduction in their serum levels. The combined therapy showed better results than methotrexate and losartan alone. Hind paws showed better improvement of inflammatory cell infiltration and bone resorption in the combined therapy group. Disturbances in liver architecture and fibrosis caused by adjuvant arthritis were reverted to normal status in the combined therapy group in contrast to losartan and methotrexate monotherapies. In conclusion, methotrexate and losartan combined therapy provided more effective anti-inflammatory and hepatoprotective effects than either drug alone.
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The relevance of animal models of rheumatoid arthritis and their relationship to the development of antiarthritic therapies is reviewed in depth. Different mechanisms for the induction of experimental arthritis, including infectio...
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The relevance of animal models of rheumatoid arthritis and their relationship to the development of antiarthritic therapies is reviewed in depth. Different mechanisms for the induction of experimental arthritis, including infectious processes, non-specific inflammation, autoimmune responses to cartilage components, and genetic manipulation are discussed in context of pathological pathways relevant to rheumatoid arthritis. A variety of species, including rats, mice, dogs, pigs, and monkey are examined for advantages and drawbacks in preclinical development of anti-arthritic agents, and their capacity to mimic critical aspects of arthritis pathology. The history of anti-arthritic therapy from non-steroidal anti-inflammatory agents to biological response modifiers is placed in context with the evolution of animal models of arthritis. The potential of novel models based upon targeted gene manipulations and corresponding pathway-specific drugs is examined as a new approach to identifying therapies relevant to rheumatoid disease.
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Rheumatoid arthritis (RA) is a systemic inflammatory disease. C-C chemokine receptor type 5 (CCR5) is found in inflamed synovium of RA patients and is necessary for formation of RA. We aimed to check whether delivery of CCR5-speci...
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Rheumatoid arthritis (RA) is a systemic inflammatory disease. C-C chemokine receptor type 5 (CCR5) is found in inflamed synovium of RA patients and is necessary for formation of RA. We aimed to check whether delivery of CCR5-specific small interfering RNA (siRNA) via electroporation suppresses local inflammation in arthritis rats. Vectors encoding siRNA that target CCR5 or negative control siRNA were constructed for gene silencing and the silencing effects of suppressing CCR5 expression in synovium examined by western blot. The vector with strongest effect was delivered into the knee joint of adjuvant-induced arthritis (AIA) rats by the in vivo electroporation method 7, 10, 13, and 16 days after immunization with Complete Freund's adjuvant. During an observation of 28 days, behavior, paw swelling, arthritis and histopathologic scoring were estimated. The expression level of CCR5 in synovium was evaluated by western blot and real-time PCR. Anti-CCR5 D1 siRNA was effectively inhibited CCR5 expression in vitro. Moreover, delivery of the siRNA into inflammatory joint also suppressed the expression of CCR5 in vivo and markedly suppressed paw swelling and inflammation. Local electroporation of anti-CCR5 siRNA into the left inflamed joints could achieve the silencing of CCR5 gene and alleviate local inflammation just in the knee joint injected with siRNA other than the opposite joint. Inhibition of CCR5 expression may provide a potential for treatment of RA. (C) 2014 Elsevier B.V. All rights reserved.
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Rheumatoid arthritis (RA) is a chronic autoimmune disease of idiopathic etiology that triggers inflammatory cytokines compromising the joint mobility. Epidemiological evidences recommend the utilization of galantamine (GH) to reve...
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Rheumatoid arthritis (RA) is a chronic autoimmune disease of idiopathic etiology that triggers inflammatory cytokines compromising the joint mobility. Epidemiological evidences recommend the utilization of galantamine (GH) to reverse the anti-inflammatory reactions induced RA. Oral administration of GH is non-selectivity due to its association with serious gastrointestinal symptoms which, could hinder its therapeutic success. Therefore, the present study aimed to validate the therapeutic potential of GH transdermal patches as a novel application to constitute an effective and tolerable delivery system for managing RA in adjuvant arthritis model. RA was induced in Sprague-Dawley rats intradermally by Heat-killed M (0.12 ml/day). Oral GH (1.25 mg/kg/day) and GH transdermal patch (2.5 mg/kg/2 days) were administrated for 14 days, during which the hind paw and body weight (BW) were assessed. Effects of C-reactive protein (CRP), inflammatory cytokines (TNF-alpha, IL-10 and IL-1 beta) and Janus kinase (JAK-2) were evaluated. Oral- and transdermal GH significantly improved the hind paw edema in arthritis animal model and offered a protective impact against RA. Oral GH group showed marked decrease in BW than that of transdermal patches group. Transdermal patch group showed a significant decrease in the level of IL-1 beta more than the oral group. However, no significant difference was detected in the levels of TNF-alpha and IL-10 between the two groups. It is concluded that GH transdermal patch can be a promising drug delivery system that copes with side effects better than oral GH consequently represents novel strategy in management of RA.
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Objective The aim of this study was to evaluate the progression of arthritis in a complete Freund’s adjuvant (CFA)-induced Wistar rat model and to monitor inflammatory arthritis activity using thermal i...
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Objective The aim of this study was to evaluate the progression of arthritis in a complete Freund’s adjuvant (CFA)-induced Wistar rat model and to monitor inflammatory arthritis activity using thermal imaging compared with histopathology.
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Abstract Ardisia solanacea is a well-known herb with a significant medicinal value. Traditionally, the plant extracts have commonly been used as an immunomodulator, antimicrobial, thrombolytic, anthelminthic, and cytotoxic agent i...
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Abstract Ardisia solanacea is a well-known herb with a significant medicinal value. Traditionally, the plant extracts have commonly been used as an immunomodulator, antimicrobial, thrombolytic, anthelminthic, and cytotoxic agent in India. The objective of this study was to assess the in-vivo antiarthritic potential of A. solanacea in the adjuvant-induced arthritic rat model. The rats were divided into nine groups (n?=?6). They were administered with complete freunds adjuvant (CFA) in the right hind paw and treated with different doses (250?mg/kg, 500?mg/kg) of the leaf extract orally from day one to day 21st. The standard group was treated with 10?mg/kg body weight of diclofenac sodium. Throughout the treatment, we monitored the differences in paw edema, body weight (BW), and arthritis index in different groups of animals. Besides, we also have evaluated the tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, C-reactive protein, rheumatoid factor (RF), and hematology. Further, we have assessed the severity of arthritis through radiography and histopathology of ankle joints. In addition, we also have studied liver and kidney histology. The arthritic symptoms were subsided significantly (p < 0.01) in the test extract treated groups at a higher dose. We also observed a downregulation of cytokines in the ASM and ASW treated group compared to the negative control. Furthermore, radiography and histopathology revealed improved prognosis in ASM and ASW administered groups. Methanolic and aqueous extract of Ardisia solanacea exhibited potential antiarthritic effect against CFA-induced arthritic rats.Graphic abstract
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CCDC134 might be an immune cytokine and plays important and complex roles in the process in vivo. It was proved to illustrate its potent antitumor effects by augmenting CD8(+) T-cell mediated immunity, but its role in the developm...
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CCDC134 might be an immune cytokine and plays important and complex roles in the process in vivo. It was proved to illustrate its potent antitumor effects by augmenting CD8(+) T-cell mediated immunity, but its role in the development of rheumatoid arthritis (RA) remains unclear. In this study, we demonstrated that development of adjuvant-induced arthritis and pro-inflammatory responses were more ameliorated in CCDC134-overexpressing transgenic mice than those in WT mice. The underlying mechanism of CCDC134-induced effects involved inhibition of T helper (Th) 1 and Th17 cell differentiation. These findings indicate that overexpression of CCDC134 exerts potent anti-inflammatory effects through selective modulation of pathogenic Th1 and Th17 cells, and might provide insights into the role of CCDC134 as a unique therapeutic agent for the treatment of rheumatoid arthritis. (C) 2017 Elsevier Inc. All rights reserved.
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